• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Activity

  Recombinant Human HA-LC3/MAP1LC3A/Apg8p3 AMC is a fluorogenic substrate for some Apg-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 380 nM and an emission wavelength of 460 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-LC3/MAP1LC3A/Apg8p3 AMC concentration of 0.1-1 μM.

• Source

  E. coli-derived

• Accession #

Background: LC3A

• Long Name:

  Microtubule-associated Protein 1 Light Chain 3 alpha

• Entrez Gene IDs:

  84557 (Human); 66734 (Mouse); 362245 (Rat); 852200 (Yeast)

• Alternate Names:

  Apg8; APG8a; Apg8p3; ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; lc3-i, ii; MAP1A/1B light chain 3 A; MAP1ALC3; MAP1ALC3MAP1A/MAP1B LC3 A; MAP1BLC3MAP1A/MAP1B light chain 3 A; MAP1LC3A; microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; MLP3A