• Species Reactivity

  Human

• Specificity

  Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP?2.

• Source

  Monoclonal Mouse IgG    _2B Clone # 66119

• Purification

  Protein A or G purified from ascites

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human BMP?4    
  Ser293-Arg408    
  Accession # P12644

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human BMP?4 (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize BMP?4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T.     et al. (1994) J. Cell Biol.     127:1755. The Neutralization Dose (ND    ₅₀) is typically 1-3 μg/mL in the presence of 500 ng/mL Recombinant Human BMP?4 and 2 μg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

• References:

1. Zhang, P. et al., 2008, Blood 111:1933.

2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.

3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.

4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.

5. Frank, D.B. et al., 2005, Circ. Res. 97:496.

6. Wozney, J. et al., 1988, Science 242:1528.

7. Cui, Y. et al., 1998, EMBO J. 17:4735.

8. Cui, Y. et al., 2001, Genes Dev. 15:2797.

9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.

10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.

11. Chen, D. et al., 2004, Growth Factors 22:233.

12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.

13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.

14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.

15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

• Long Name:

  Bone Morphogenetic Protein 4

• Entrez Gene IDs:

  652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

• Alternate Names:

  BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME