• Species Reactivity

  Human, Mouse

• Specificity

  Stains human Jagged 1 and Jagged 2 transfectants but not the parental cell line by flow cytometry. Detects endogenous human and mouse Jagged 1 and Jagged 2 by flow cytometry.

• Source

  Monoclonal Mouse IgG    _2B Clone # 241002

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Jagged 2    
  Met27-Asp307    
  Accession # Q9Y219

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Jagged 1/Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

• References:

1. Shawber, C. et al. (1996) Dev. Biol. 180:370.

2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.

3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.

4. Schickwann, T. et al. (2000) Blood 96:950.

5. DeHart, S. et al. (2005) Blood 105:3521.

6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

• Entrez Gene IDs:

  182 (Human)

• Alternate Names:

  Jagged 1/Jagged 2