• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).

• Source

  Monoclonal Rat IgG    _2B Clone # 30-F11

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse thymus and spleen cells

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Mouse splenocytes

• 
  

• Immunohistochemistry

  Simon, D.I.     et al. (2000) J. Clin. Invest.     105:293.

• 
  

• Immunoprecipitation

  Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev.     47:63.

• 
  

• Complement-dependent Cytotoxicity

  Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev.     47:63.

• 
  

• CyTOF-reported

  Lee, H.     et al. (2015) Mucosal Immunol.     8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180?220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1?3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1?5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode
aa 30?169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6?8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8?10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11?14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

• References:

1. Anderson, J.N. et al. (2004) FASEB J. 18:8.

2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.

3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.

4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.

5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.

6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.

7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.

8. McNiell, L. et al. (2007) Immunity 27:425.

9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.

10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.

11. Zhu, J.W. et al. (2008) Immunity 28:183.

12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.

13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.

14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.

15. Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

• Long Name:

  Cluster of Differentiation 45

• Entrez Gene IDs:

  5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)

• Alternate Names:

  B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide