• Species Reactivity

  Human

• Specificity

  Detects human Caveolin-3 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 682708

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Caveolin-3    
  Met1-Pro83    
  Accession # P56539

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 μg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caveolin-3

Caveolin-3 is an 18 kDa member of the Caveolin family of proteins which are major protein components of plasma membrane caveolae. It is preferentially expressed in muscle cells where it regulates the localization and activity of potassium channels and the nicotinic acetylcholine receptor. Caveolin-3 mutations are associated with several skeletal muscle defects. It contains a central membrane-embedded sequence (aa 84-104) flanked by two cytoplasmic domains (aa 1-83 and 105-151). Over aa 1-83, human Caveolin-3 shares 94% aa identity with mouse and rat Caveolin-3.

• Entrez Gene IDs:

  859 (Human); 12391 (Mouse); 29161 (Rat)

• Alternate Names:

  CAV3; caveolin 3; Caveolin3; Caveolin-3; LGMD1C; LGMD1CMGC126100; LQT9; LQT9MGC126101; M-caveolin; MGC126129; VIP21; VIP-21