• Species Reactivity

  Human

• Specificity

  Detects human SHARPIN in ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 881816

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SHARPIN    
  Ala27-Ala182    
  Accession # Q9H0F6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHARPIN

SHARPIN (Shank-associated RH Domain-interacting Protein), also called SIPL1 (Shank Interacting Protein-like 1) is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFk beta, thus regulating immune and inflammatory responses. SHARPIN is widely expressed, with upregulated expression in multiple tumor types. Human SHARPIN is a 387 amino acid (aa) protein containing self-association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human SHARPIN shares 67% and 66% aa sequence identity with mouse and rat SHARPIN, respectively.

• Long Name:

  SHANK-associated RH Domain Interactor

• Entrez Gene IDs:

  81858 (Human); 106025 (Mouse); 81859 (Rat)

• Alternate Names:

  DKFZp434N1923; hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; SHARPIN; SIPL1; SIPL1DKFZP434N1923