• Species Reactivity

  Mouse

• Specificity

  Detects mouse TLR4 in direct ELISAs.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1203B

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TLR4    
  Asn26-Lys629    
  Accession # Q9QUK6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Immunocytochemistry

  1-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

• Long Name:

  Toll-like Receptor 4

• Entrez Gene IDs:

  7099 (Human); 21898 (Mouse); 29260 (Rat)

• Alternate Names:

  ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TLR4; TOLL; toll-like receptor 4