• Species Reactivity

  Human

• Specificity

  Detects human EGF R phosphorylated at Y1068.

• Source

  Monoclonal Mouse IgG2a Clone # 338324

• Immunogen

  Phosphopeptide containing human EGF R Y1068 site

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # ), fixed with paraformaldehyde, and permeabilized with saponin

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGFR

Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

• Long Name:

  Epidermal Growth Factor Receptor

• Entrez Gene IDs:

  1956 (Human); 13649 (Mouse)

• Alternate Names:

  avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1