• Species Reactivity

  Mouse

• Specificity

  Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN? gamma, rrIFN? gamma, rpIFN? gamma, rrmIFN? gamma, rfeIFN? gamma, or rcaIFN? gamma.

• Source

  Monoclonal Rat IgG    _2A Clone # 37895

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IFN-gamma

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Mouse IFN-gamma (Catalog # )

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Neutralization

  Measured by its ability to neutralize IFN? gamma inhibition of EMCV-induced cytopathy in the L?929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND    ₅₀) is typically 0.075-0.3 μg/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN? gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype  
pro-inflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque IFN-gamma. IFN-gamma dimers bind to IFN? gamma  RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits anti-viral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

• References:

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.

2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.

3. Gray, P.W. and D.V. Goeddel (1983) Proc. Natl. Acad. Sci. USA 80:5842.

4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.

5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.

6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.

7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.

8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.

9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

• Long Name:

  Interferon gamma

• Entrez Gene IDs:

  3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

• Alternate Names:

  IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma