詳細(xì)說明
Species Reactivity
Mouse
Specificity
Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
Source
Monoclonal Rat IgG 2B Clone # 247506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25 μg/10 6 cells
Mouse thymocytes
Immunohistochemistry
8-25 μg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 μg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL12/SDF?1 alpha -induced chemotaxis in the BaF3 mouse pro?B cell line transfected with mouse CXCR4. The Neutralization Dose (ND 50) is typically 8?40 μg/mL in the presence of 10 ng/mL Recombinant Mouse CXCL12/SDF?1 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Neutralization | Chemotaxis Induced by CXCL12/SDF?1 alpha and Neutralization by Mouse CXCR4 Antibody. Recombinant Mouse CXCL12/SDF?1 alpha (Catalog # ) chemo-attracts the BaF3 mouse pro?B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # ). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF?1 alpha (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND50 is typically 8-40 μg/mL. |
Immunocytochemistry | CXCR4 in Mouse Splenocytes. CXCR4 was detected in immersion fixed mouse splenocytes using 10 μg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights? 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). View our protocol for . |
Immunocytochemistry | CXCR4 in Mouse Neural Progenitor Cells. CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 μg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights? 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). View our protocol for . |
Immunohistochemistry | CXCR4 in Mouse Spleen. CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 μg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR4
CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.
Entrez Gene IDs:
7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names:
CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
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