• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-1 alpha /IL-1F1 in ELISAs. In ELISAs, less than 1% cross-reactivity with recombinant rat IL?1 alpha /IL?1F1, recombinant porcine    
  IL?1 alpha /IL?1F1, and recombinant human IL?1 alpha /IL?1F1 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 40508

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IL-1 alpha /IL-1F1    
  Ser115-Ser270    
  Accession # P01582

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Mouse IL-1 alpha /IL-1F1 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Mouse IL?1 alpha /IL?1F1 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Mouse IL?1 alpha /IL?1F1 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse IL-1 alpha/IL-1F1 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IL?1 alpha /IL?1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A.      et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J.      et al. (eds): IRL Press. 272. The Neutralization Dose (ND     ₅₀) is typically 0.2-0.6 μg/mL in the presence of 50 pg/mL Recombinant Mouse IL?1 alpha /IL?1F1 and 1.25 μg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta ?converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti?inflammatory agents such as glucocorticoids, IL-4, and IL-13.

• Long Name:

  Interleukin 1 alpha

• Entrez Gene IDs:

  3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)

• Alternate Names:

  Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha