• Species Reactivity

  Mouse, Rat

• Specificity

  Detects mouse and rat FABP2/I?FABP in Western blots. Detects rat FABP2/I?FABP in direct ELISAs. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat FABP1, recombinant human FABP3, recombinant mouse (rm) FABP4, and rmFABP5 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant rat FABP2/I?FABP    
  Met1-Glu132    
  Accession # P02693

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

• Immunocytochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP2/I-FABP

FABP2, also named I-FABP and gFABP, is a member of the intracellular fatty acid binding protein family. It is highly expressed in the intestine. FABP2 binds fatty acid in a non-covalent 1:1 complex to chaperone the lipids to cellular enzymes for metabolism and signal transduction.

• Long Name:

  Fatty Acid-Binding Protein 2/Intestinal FABP

• Entrez Gene IDs:

  2169 (Human); 25598 (Rat)

• Alternate Names:

  FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein