• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit TWEAK-induced apoptosis in HT?29 human colon adenocarcinoma cells. The ED    ₅₀ for this effect is 2-12 μg/mL in the presence of 1 μg/mL recombinant human TWEAK. Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED    ₅₀ for this effect is 10-30 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.

• Source

  Mouse myeloma cell line, NS0-derived

  Human TWEAK R (Glu28-Trp79) Accession # Q9NP84	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu28

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  32 kDa (monomer)

• SDS-PAGE

  35-45 kDa, reducing conditions

Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1 - 3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2 - 6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

• References:

1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.

2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.

3. Wiley, S. et al. (2001) Immunity 15:837.

4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.

5. Nakayama, M. et al. (2002) J. Immunol. 168:734.

6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

• Long Name:

  TNF-like and Weak Inducer of Apoptosis Receptor

• Entrez Gene IDs:

  51330 (Human); 27279 (Mouse)

• Alternate Names:

  CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14