• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M.     et al. (2005) J. Biol. Chem.     280:15842. The ED    ₅₀ for this effect is 150-600 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Ser27-Pro524, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser27

• Predicted Molecular Mass

  55.6 kDa

• SDS-PAGE

  60-70 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Data Images

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

• References:

1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.

2. Iso, Tatsuya, et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.

3. Walker, L., et al. (2001) Stem Cells 19:543.

4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.

5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.

6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

• Long Name:

  Delta-like 4

• Entrez Gene IDs:

  54567 (Human); 54485 (Mouse)

• Alternate Names:

  Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4