• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M.     et al. (2005) J. Biol. Chem.     280:15842. The ED    ₅₀ for this effect is 0.25-1.0 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human DLL1 (Ser22-Gly540) & (His152-Gly540) Accession # AAG09716	DI	6-His tag
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser22 & His152

• Predicted Molecular Mass

  57 kDa and 43 kDa

• SDS-PAGE

  66 kDa and 55 kDa, reducing conditions

Background: DLL1

Delta-like protein 1 (DLL1) is a 90 - 100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6 - 11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

• References:

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 

2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 

3. Karanu, F.N. et al. (2001) Blood 97:1960. 

4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 

5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 

6. Takahashi, Y. et al. (2003) Development 130:4259. 

7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.

8. Kiernan, A.E. et al. (2005) Development 132:4353.

9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.

10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.

11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.

12. Limbourg, A. et al. (2007) Circ. Res. 100:363.

13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

• Long Name:

  Delta-like 1

• Entrez Gene IDs:

  28514 (Human); 13388 (Mouse); 84010 (Rat)

• Alternate Names:

  delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1