詳細說明
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its anti-microbial activity against E. coli. Ganz, T. et al. (2003) Nat. Rev. Immunol. 3:710. The ED 50 for this effect is 7.5-30 μg/mL. Measured by its ability to promote internalization of cell surface CXCR4 on Jurkat human acute T cell leukemia cells. Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39. As determined by flow cytometric analysis, the effect is a 2?3 fold reduction in fluorescence intensity of Jurkat cells stained with a CXCR4 antibody (Catalog # ) following a 2 hour pre-treatment with 10 μg/mL human
beta -Defensin 3 compared to untreated cells.
Optimal dilutions should be determined by each laboratory for each application.Source
E. coli-derived Gly23-Lys67
Accession #
N-terminal Sequence
AnalysisGly23
Predicted Molecular Mass
5.2 kDa
4435-BD |
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Formulation Lyophilized from a 0.2 μm filtered solution in HCl. | ||
Reconstitution Reconstitute at 500 μg/mL in sterile 4 mM HCl. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: beta-Defensin 3
beta -Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 45 amino acid (aa) mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively (2, 3). It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4-6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4 (5, 13), and with HSV glycoprotein B plus its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15).
References:
Dhople, V. et al. (2006) Biochim. Biophys. Acta 1758:1499.
Harder, J. et al. (2001) J. Biol. Chem. 276:5707.
Schibli, D.J. et al. (2002) J. Biol. Chem. 277:8279.
Garcia, J.-R.C. et al. (2001) Cell Tissue Res. 306:257.
Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39.
Sorensen, O.E. et al. (2006) J. Clin. Invest. 116:1878.
Varoga, D. et al. (2005) Arthritis Rheum. 52:1736.
Taggart, C.C. et al. (2003) J. Immunol. 171:931.
Joly, S. et al. (2004) J. Clin. Microbiol. 42:1024.
Chen, X. et al. (2007) Eur. J. Immunol. 37:434.
Kluver, E. et al. (2005) Biochemistry 44:9804.
Wu, Z. et al. (2003) Proc. Natl. Acad. Sci. 100:8880.
Feng, Z. et al. (2006) J. Immunol. 177:782.
Hazrati, E. et al. (2006) J. Immunol. 177:8658.
Leikina, E. et al. (2005) Nat. Immunol. 6:995.
Entrez Gene IDs:
55894 (Human)
Alternate Names:
BD14; BD3; BD-3; beta-defensin 103; betaDefensin 3; beta-Defensin 3; DEFB103; DEFB103A; DEFB103B; DEFB3; DEFB-3; DEFB3DEFB103A; Defensin, beta 103; defensin, beta 103B; Defensin-like protein; HBD-3; HBD3HBP3