• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit TSLP-dependent proliferation of BaF3 mouse pro?B cells co-transfected with human IL-7 R alpha and human TSLP R. The ED    ₅₀ for this effect is 10-30 μg/mL in the presence of 0.2 ng/mL of recombinant human TSLP.

• Source

  Mouse myeloma cell line, NS0-derived

  Human TSLP R (Gly25-Lys231) Accession #Q9HC73	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Gly25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  50.6 kDa (monomer)

• SDS-PAGE

  65-75 kDa, reducing conditions

Background: TSLP R

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3 - 6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c⁺ dendritic cells (5).

• References:

1. Fujio, K. et al. (2000) Blood 95:2204.

2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.

3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.

4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.

5. Reche, P.A. et al. (2001) J. Immunol. 167:336.

6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

• Long Name:

  Thymic Stromal Lymphopoietin Receptor

• Entrez Gene IDs:

  64109 (Human); 57914 (Mouse); 171499 (Rat)

• Alternate Names:

  CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR