• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using BaF3 mouse pro?B cells transfected with human FGF RIIIc. The ED    ₅₀ for this effect is 0.025-0.1 μg/mL in the presence of Recombinant Human FGF?23 (Catalog # ) and Heparin.

• Source

  Mouse myeloma cell line, NS0-derived Glu34-Ser981, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Glu34

• Predicted Molecular Mass

  109.8 kDa

• SDS-PAGE

  120-130 kDa, reducing conditions

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and a 10 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. A 130 kDa form found in plasma and cerebrospinal fluid is produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A truncated 549 aa isoform predicted by alternative mRNA splicing has been detected in human and mice (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF-23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)₂D₃ (vitamin D) (7). Klotho deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D
(2-7). Both Klotho and Klotho beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).

• References:

1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.

2. Kuro-o, M. et al. (1997) Nature 390:45.

3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.

4. Imura, A. et al. (2004) FEBS Lett. 565:143.

5. Kurosu, H. et al. (2005) Science 309:1829.

6. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.

7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.

8. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.

9. Chang, Q. et al. (2005) Science 310:490.

10. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.

11. Liu, H. et al. (2007) Science 317:803.

12. Imura, A. et al. (2007) Science 316:1615.

• Entrez Gene IDs:

  9365 (Human); 16591 (Mouse)

• Alternate Names:

  EC 3.2.1; EC 3.2.1.31; KL; Klotho