• Species Reactivity

  Human

• Specificity

  Stains human MCT1/SLC16A1 transfected cells but not irrelevant transfectants.    
   

• Source

  Monoclonal Mouse IgG    _2A Clone # 882616

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human MCT1/SLC16A1    
  Met1-Val500    
  Accession # P53985

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCT1/SLC16A1

Solute Carrier, family 16, member 1 (SLC16A1) encodes Monocarboxylic Acid Transporter 1 (MCT1).  MCT1 is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. MCT1 has been found to be important in lactate uptake in cancer cells, and blockade of MCT1 may inhibit lactate-induced HIF-1 activation (1).

• References:

1. Sonveaux, P. et al (2012) PLoS ONE 7:e33418.

• Long Name:

  Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

• Entrez Gene IDs:

  6566 (Human); 20501 (Mouse); 25027 (Rat)

• Alternate Names:

  HHF7; malignant T cell amplified sequence 1; Malignant T-Cell-Amplified Sequence 1; MCT1; MCT-1; Multiple Copies T-Cell Malignancies; SLC16A1