• Species Reactivity

  Human, Mouse

• Specificity

  Recognizes a carbohydrate epitope of SSEA-3 (4, 5).

• Source

  Monoclonal Rat IgM Clone # MC-631

• Purification

  IgM-specific Affinity-purified from hybridoma culture supernatant

• Immunogen

  Four to eight cell stage mouse embryos

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

• References:

1. Zhou, D.         et al. (2000) J. Biol. Chem.         275:22631.

2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol.         18:53.

3. Draper, J.S.         et al. (2002) J. Anat.         200:249.

4. Shevinsky, L.H.         et al. (1982) Cell         30:697.

5. Kannagi, R. et al. (1983) EMBO J. 2:2355.

• Long Name:

  Stage-specific Embryonic Antigen-3

• Alternate Names:

  SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3