• Species Reactivity

  Human

• Specificity

  Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF? beta 1, rhTGF? beta 2, rhTGF? beta 1.2, rhTGF? beta 3, or rhTGF? alpha.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 27232

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human LAP (TGF-beta 1)    
  Leu30-Arg278 (Cys33Ser)    
  Accession # P01137

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAP (TGF-beta 1)

TGF-beta 1 is one member of a six gene family that has been described in mammals, birds, fish and frog. The name TGF-beta is applied to a 24-28 kDa disulfide-linked dimer that is generated through the proteolytic processing of a larger precursor molecule. For TGF-beta 1, a 50-55 kDa, 391 amino acid (aa) proprecursor is first, covalently linked to a second proprecursor (creating a disulfide-linked homodimer), and second, internally cleaved to generate two covalently-linked homodimers that remain non-covalently associated. The smallest homodimer representing aa 279-390 of the proform is TGF-beta 1; the largest homodimer representing aa 30-278 of the proform is termed LAP (Latency-associated Peptide). The LAP homodimer wraps itself around the smaller TGF-beta 1 homodimer, thus blocking an interaction of mature TGF-beta with its receptors. Almost all cells secrete the inactive TGF-beta :LAP complex, and most do so with LAP covalently bound to a very large 120-160 kDa LTBP (Latent TGF-beta Binding Protein), platelets being a notable exception. LTBP associates with multiple matrix components and this serves to store TGF-beta extracellularly in a non-active form. When TGF-beta signaling is needed, the LTBP:matrix association is disrupted, and the TGF-beta :LAP complex is exposed to multiple LAP binding partners such as TSP-1 and various Integrins. Interactions with these factors cause LAP to unwrap and dissociate from TGF-beta, resulting in TGF-beta "activation" and receptor binding. Ther are three human TGF-beta 1 LAPs and TGF-beta 1 LAP shares 36% and 33% aa sequence indentity with TGF-beta 2 LAP and TGF-beta 3 LAP, respectively. Human to mouse, TGF-beta 1 LAP shares 86% aa sequence identity. 

• Long Name:

  Latency-associated Peptide

• Entrez Gene IDs:

  7040 (Human)

• Alternate Names:

  LAP (TGFbeta 1); LAP (TGF-beta 1)