• Species Reactivity

  Mouse

• Specificity

  Detects mouse TIGIT in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human TIGIT is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TIGIT    
  Gly26-Thr143    
  Accession # NP_001139797

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to either PVR/CD155 or PVRL2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, TIGIT and CD226/DNAM1 pairings with PVR and PVRL2 appear to form a network that resembles the well-characterized B7-1 and B7-2 pairings with CD28 and CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity and IFN-gamma production. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is unusual in that it shows an addition of nine amino acids spread over three insertion sites. Over aa 26-143, mouse TIGIT shares only 84% aa sequence identity with rat TIGIT, but only 68% aa sequence identity with human TIGIT.

• Long Name:

  T Cell Immunoreceptor with Ig and ITIM Domains

• Entrez Gene IDs:

  201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

• Alternate Names:

  TIGIT; VSIG9; VSTM3; WUCAM