詳細(xì)說明
Species Reactivity
Human
Specificity
Detects human HNF?3 beta /FoxA2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HNF?3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Immunocytochemistry
10 μg/mL
See below
Intracellular Staining by Flow Cytometry
5 μL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry | HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells. BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # ). HNF-3 beta /FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 μg/mL Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor? 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for . |
Intracellular Staining by Flow Cytometry | Detection of HNF?3 beta /FoxA2 in Human iPS Cells by Flow Cytometry. Human iPS cells were stained with Goat Anti-Human HNF?3 beta /FoxA2 Alexa Fluor? 488?conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: HNF-3 beta/FoxA2
HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.
Long Name:
Hepatocyte Nuclear Factor-3, beta
Entrez Gene IDs:
3170 (Human)
Alternate Names:
forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B