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Human HNF-3 beta/FoxA2 Alexa Fluor 488 Aff Pur Polyclonal Ab 100 TESTS

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human HNF?3 beta /FoxA2 in direct ELISAs and Western blots.

    • Source

      Polyclonal Goat IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human HNF?3 beta /FoxA2    
      Met242-Ser457    
      Accession # Q9Y261

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 488

    Applications


    • Recommended    
      Concentration

      Sample

    • Immunocytochemistry

      10 μg/mL

      See below


    • Intracellular Staining by Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Immunocytochemistry      
         

    HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells. BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # ). HNF-3 beta /FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 μg/mL Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor? 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for .

    Intracellular Staining by Flow Cytometry      
         

    Detection of HNF?3 beta /FoxA2 in Human iPS Cells by Flow Cytometry. Human iPS cells were stained with Goat Anti-Human HNF?3 beta /FoxA2 Alexa Fluor? 488?conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: HNF-3 beta/FoxA2

    HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

    • Long Name:

      Hepatocyte Nuclear Factor-3, beta

    • Entrez Gene IDs:

      3170 (Human)

    • Alternate Names:

      forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B









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