• Species Reactivity

  Human

• Specificity

  Detects human Carboxypeptidase X1/CPXM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross?reactivity with recombinant mouse CPXM is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase X1/CPXM1    
  Ala21-Asp734    
  Accession # Q96SM3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human Carboxypeptidase X1/CPXM1

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase X1/CPXM1,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase X1/CPXM1

Carboxypeptidases cleave amino acids from the C-terminus of proteins and peptides and many are metalloproteases. They have distinct expression patterns and different specificities for example, preferentially cleaving aromatic (carboxypeptidase As) or basic (carboxypeptidase Bs) residues. Several, such as carboxypeptidase Xs, have lost their catalytic activity. Carboxypeptidases play important roles in digestion of food, processing of bioactive peptides and blood coagulation.

• Entrez Gene IDs:

  56265 (Human); 56264 (Mouse)

• Alternate Names:

  carboxypeptidase X (M14 family); carboxypeptidase X (M14 family), member 1; Carboxypeptidase X1; CPX1; CPX-1; CPX1EC 3.4.17.-; CPXM; CPXM1; EC 3.4.17; EC 3.4.17.10; EC 3.4.17.3; Metallocarboxypeptidase CPX-1; probable carboxypeptidase X1