• Species Reactivity

  Human

• Specificity

  Detects human Complement Factor H-related 1/CFHR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross?reactivity with recombinant human (rh) FHR2 is observed and approximately 15% cross-reactivity with rhFHR5 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 1/CFHR1    
  Glu19-Ala328    
  Accession # Q03591

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human Complement Factor H-related 1/CFHR1

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Human    
  Complement Factor H-related 1/CFHR1,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 1/CFHR1

FHR1 (Complement Factor H-related protein 1) is a 43 kDa, secreted member of the factor H family of glycoproteins. It is produced by hepatocytes and circulates as two differentially glycosylated isoforms (37 kDa and 43 kDa). Mature human FHR1 is 312 amino acids (aa) in length. It contains five, approximately 60 aa SCRs (short consensus repeats/CCPs/SUSHI repeats) that basically constitute the entire molecule. FHR1 may play a role in lipoprotein complexes that bind PMNs to LPS. There is no reported rodent counterpart to FHR1. Over aa 19?143 of the FHR1 precursor, human FHR2 and FHR5 show 98% and 86% aa identity, respectively.

• Entrez Gene IDs:

  3078 (Human)

• Alternate Names:

  BTA; CFHL1FHR-1; CFHLCFHR1P; CFHR1; complement factor H-related 1 pseudogene; Complement Factor Hrelated 1; Complement Factor H-related 1; complement factor H-related protein 1; FHR1; FHR1CFHL1P; H factor (complement)-like 1; H factor (complement)-like 2; H factor-like protein 1; H36-1; H36-2; H-factor-like 1; HFL1H36; HFL2; MGC104329