• Species Reactivity

  Human

• Specificity

  Detects human Aminopeptidase O/ONPEP in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Aminopeptidase O/ONPEP isoform 1    
  Met1-Phe819    
  Accession # Q8N6M6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human Aminopeptidase O/ONPEP

• 
  

• Immunohistochemistry

  5-15 μg/mL

  Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # )

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase O/ONPEP,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase O/ONPEP

Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it cleaves N-terminal Arg and Asn residues. Human AP-O is 819 amino acids in length. Aminopeptidase O contains one catalytic domain (aa 167 - 589) with a zinc-binding site (His479 - His483) and a C-terminal ARM region (aa 706 - 819). At least three additional isoforms of Aminopeptidase O exist. Full-length human AP-O is 76% aa identical to mouse AP-O.

• Entrez Gene IDs:

  84909 (Human); 72061 (Mouse); 290963 (Rat)

• Alternate Names:

  Aminopeptidase O; AOPEP; APO; AP-OFLJ55832; C90RF3; C9orf3; chromosome 9 open reading frame 3; EC 3.4.11.-; FLJ14675; FLJ40923; ONPEP