• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q in direct ELISAs and Western blots.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human T Cell Receptor alpha Chain-V alpha 24-J alpha Q    
  Lys22-Pro135    
  Accession # ABC72391

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: T Cell Receptor alpha Chain-V alpha 24-J alpha Q

V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKT^INV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco?/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36?133) and a TCR-related sequence of unknown function (aa 142?255). Considerable variability is noted over aa 22?114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22?135.

• Alternate Names:

  T Cell Receptor alpha Chain-V alpha 24-J alpha Q; T CellReceptor alpha ChainV alpha 24J alpha Q