• Species Reactivity

  Human

• Specificity

  Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Adenosylhomocysteinase/AHCY    
  Ser2-Tyr432    
  Accession # P23526

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosylhomocysteinase/AHCY

Human S?Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co?factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S?adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S?adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S?adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).

• References:

1. Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101.

2. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670.

3. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942.

4. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234.

5. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347. 

• Entrez Gene IDs:

  191 (Human)

• Alternate Names:

  Adenosylhomocysteinase; AdoHcyase; AHCY; EC 3.3.1.1; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHHadoHcyase