詳細(xì)說(shuō)明
Species Reactivity
Mouse
Specificity
Detects mouse Mast Cell Protease?1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Mcpt6, rmMcpt7, and rmMcpt11 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease?1/Mcpt1
Glu19-Leu246
Accession # P11034Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 μg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Mouse Mast Cell Protease?1/Mcpt1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 μg/mL of Mouse Mast Cell Protease?1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Mast Cell Protease?1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mast Cell Protease-1/Mcpt1
Mast cell protease-1 (Mcpt1), also known as beta ?chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMcpt1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N?terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha ?Chymase, Mcpt1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.
References:
Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.
Entrez Gene IDs:
17224 (Mouse); 29265 (Rat)
Alternate Names:
AV080368; Mast Cell Protease1; Mast Cell Protease-1; Mcp1; Mcp-1; MMPC-1