• Species Reactivity

  Mouse, Rat

• Specificity

  Detects mouse and rat Neuroglycan C/CSPG5 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Neuroglycan C is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse Neuroglycan C/CSPG5    
  Val31-Gln420    
  Accession # AAH55736

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1?2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2-3). Mouse NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 32-273), with CS attachment at Ser117, one EGF-like domain (aa 371-413), three potential sites for N-linked glycosylation, and ten potential sites for O-linked glycosylation (4). Splicing variants produce four isoforms for human NGC. Isoform 1 is the standard form. Isoform 2 has a deletion of aa 487-513, while isoform 3 has an alternative start site at Met82 and the same deletion. Isoform 4 has a 56 aa substitution for aa 514-566. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31-420, mouse NGC shares 84% aa identity with human NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ErbB3 tyrosine kinase and transactivating ErbB2 (1).

• References:

1. Kinugasa, Y. et al. (2004) Biochem. Biophys Res. Commun 321:1045.

2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.

3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.

4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.

5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.

6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.

7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.

• Entrez Gene IDs:

  10675 (Human); 29873 (Mouse); 50568 (Rat)

• Alternate Names:

  CALEB; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; CSPG5; MGC44034; Neuroglycan C; NGC; NGCAcidic leucine-rich EGF-like domain-containing brain protein