• Species Reactivity

  C. botulinum

• Specificity

  Detects     C. botulinum BoNTA Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross?reactivity with recombinant     C. botulinum BoNTA Heavy Chain is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant Clostridium BoNT-A Light Chain    
  Met1-Phe425    
  Accession # Q45894

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Botulinum Neurotoxin Type A Light Chain (Catalog # )

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant     C. botulinum BoNT-A Light Chain (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-A Light Chain

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

• References:

1. Willems, A. et al. (1993) Res. Microbiol. 144:547.

2. Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.

3. Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.

4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

• Long Name:

  Botulinum Neurotoxin Type A Light Chain

• Entrez Gene IDs:

  5185061 (C. botulinum)

• Alternate Names:

  BoNTA Light Chain; BoNT-A Light Chain