• Species Reactivity

  Mouse

• Specificity

  Detects mouse Dectin?1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.

• Source

  Monoclonal Rat IgG2a Clone # 218820

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Dectin?1/CLEC7A    
  Phe69-Leu244    
  Accession # Q6QLQ4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  RAW 264.7 mouse monocyte/macrophage cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin?1, respectively. It shares 25%?34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8?10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11?13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

• References:

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.

2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.

3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.

4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.

5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 

6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 

7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.

8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.

9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.

10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.

11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.

12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.

13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.

14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.

15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.

16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

• Entrez Gene IDs:

  64581 (Human); 56644 (Mouse)

• Alternate Names:

  Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1