• Species Reactivity

  Human

• Specificity

  Detects human IL?17 RA/IL?17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL?17 R, rmIL?17B R, or recombinant human IL?17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

• Source

  Monoclonal Mouse IgG1 Clone # 133617

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL?17 RA/IL?17 R    
  Leu33-Trp320    
  Accession # Q96F46

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  K562 human chronic myelogenous leukemia cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17 RA/IL-17 R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL?17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

• References:

1. Yao, Z. et al. (1997) Cytokine 9:794.

2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

• Long Name:

  Interleukin 17 Receptor

• Entrez Gene IDs:

  23765 (Human); 16172 (Mouse); 312679 (Rat)

• Alternate Names:

  CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A