• Species Reactivity

  Human

• Specificity

  Detects human CCRL2/CRAM?A/B. Stains human HCR-transfected cells but not irrelevant transfectants. It detects both the CRAM-A and CRAM-B isoforms.

• Source

  Monoclonal Mouse IgG2b Clone # 152211

• Immunogen

  NS0 mouse myeloma cell line transfected with human CCRL2/CRAM?A/B    
  Met1-Val356 (Val180Met)    
  Accession # NP_001124382

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human CCRL2/CRAM-A transfected NS0 cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCRL2/CRAM-A/B

HCR, also known as CRAM-A, CRAM-B, CKRX and CCRL2, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. Two isoforms were reported and designated CRAM-A and CRAM-B (1) that differ at their N-termini by the inclusion of an additional 12 amino acids on CRAM-A. HCR/CRAM is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils and T cells (2, 3).  

• References:

1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.

2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.

• Long Name:

  CC Chemokine Receptor-like 2

• Entrez Gene IDs:

  9034 (Human)

• Alternate Names:

  ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor