• Species Reactivity

  Human, Primate

• Specificity

  Detects human NCAM?1/CD56 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 301040

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NCAM?1/CD56

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human peripheral blood mononuclear cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4?6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7?8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10?13).

• References:

1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.

2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.

3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.

4. Dickson, G. et al. (1987) Cell 50:1119.

5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.

6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.

7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.

8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.

9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.

10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.

11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.

12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.

13. Muller, D. et al. (1996) Neuron 17:413.

• Long Name:

  Neural Cell Adhesion Molecule

• Entrez Gene IDs:

  4684 (Human); 17967 (Mouse); 24586 (Rat)

• Alternate Names:

  CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM