• Species Reactivity

  Mouse

• Specificity

  Detects mouse ULBP?1 /MULT-1.

• Source

  Monoclonal Rat IgG2a Clone # 237104

• Immunogen

  BaF3 mouse pro-B cell line transfected with mouse ULBP?1 /MULT-1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Mouse ULBP?1 /MULT-1 transfected Baf/3 cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ULBP-1

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1?4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8⁺ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (K_D = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

• References:

1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.

2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.

3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.

4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.

5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.

6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.

7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

• Long Name:

  UL16 Binding Protein-1

• Entrez Gene IDs:

  80329 (Human); 77777 (Mouse)

• Alternate Names:

  ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1