• Species Reactivity

  Human

• Specificity

  Detects human LSECtin/CLEC4G in flow cytometry.

• Source

  Monoclonal Mouse IgG2a Clone # 845404

• Immunogen

  NS0 mouse myeloma cell line transfected with LSECtin/CLEC4G    
  Ser54-Cys293    
  Accession # Q6UXB4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human mature dendritic cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LSECtin/CLEC4G

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca²⁺-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1?31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil?coil neck domain (aa 96?136), a C?type lectin-like domain (CTLD) of the type found in human DC?SIGN and DC?SIGN receptor (aa 165?289), and a C-terminal Ca²⁺-dependent carbohydrate?recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca²⁺-dependent manner (1?3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin?mediated infection of cells by Ebola virus has been demonstrated (3, 4).

• References:

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.

2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.

3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.

4. Gramberg, T. et al. (2005) Virology 340:224.

• Long Name:

  Liver And Lymph Node Sinusoidal Endothelial Cell C-type Lectin

• Entrez Gene IDs:

  339390 (Human); 75863 (Mouse)

• Alternate Names:

  CLEC4G; C-type lectin domain family 4 member G; C-type lectin domain family 4, member G; C-type lectin superfamily 4, member G; DTTR431; liver and lymph node sinusoidal endothelial cell C-type lectin; LP2698; LSECtin; Q6UXB4; UNQ431