• Species Reactivity

  Human

• Specificity

  Detects human 5T4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5T4 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 524744

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human 5T4    
  Ser31-Ser355    
  Accession # Q13641

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5T4

Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32?355) that shows one Ser?rich region followed by seven Leu-rich repeats (aa 90?355). Over aa 31?355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.

• Long Name:

  Oncofetal Antigen/Trophoblast Glycoprotein

• Entrez Gene IDs:

  7162 (Human); 21983 (Mouse); 83684 (Rat)

• Alternate Names:

  5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4; 5T4AG; 5T4-AG; 5T4M6P1,5T4 oncofetal antigen; M6P1; TPBG; trophoblast glycoprotein