• Species Reactivity

  Human

• Specificity

  Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 5334

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human IL?2    
  Ala21-Thr153    
  Accession # NP_000577

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma _c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4⁺ and CD8⁺ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8⁺ T cell proliferation, and promote NK cell and CD8⁺ T cell effector activity. IL-2 also appears to drive CD4⁺ Fox P3^- thymocytes into mature FoxP3⁺ Tregs, and to direct the conversion of CD4⁺ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

• Long Name:

  Interleukin 2

• Entrez Gene IDs:

  3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

• Alternate Names:

  aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF