• Species Reactivity

  Human

• Specificity

  Detects human Dectin?2/CLEC6A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Dectin-2 alpha, recombinant human (rh) CLEC9A, rhCLEC4D, or rhDLEC is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 545943

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Dectin?2/CLEC6A    
  Thr46-Leu209    
  Accession # Q6EIG7

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Dectin-2/CLEC6A

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4⁺ T cells (4?6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up?regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4⁺CD25⁺ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

• References:

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.

2. Kanazawa, N. et al. (2004) Immunobiology 209:179.

3. Flornes, L.M. et al. (2004) Immunogenetics 56:506.

4. Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.

5. Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.

6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.

7. McGreal, E.P. et al. (2006) Glycobiology 16:422.

8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.

9. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.

10. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

• Entrez Gene IDs:

  93978 (Human); 56620 (Mouse)

• Alternate Names:

  CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2