• Species Reactivity

  Human

• Specificity

  Detects human ICOS in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 669222

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail    
  Glu21-Phe141    
  Accession # Q9Y6W8

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICOS

Inducible Costimulator (ICOS), also known as AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule), CD278, and CRP-1 (CD28-Related Protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Over aa 21-141, human and mouse ICOS share approximately 70% aa sequence identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

• Long Name:

  Inducible Co-stimulator

• Entrez Gene IDs:

  29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

• Alternate Names:

  Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850