• Species Reactivity

  Human

• Specificity

  Detects human Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interference with recombinant mouse Dtk, recombinant human (rh) Axl, or rhMer is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 96201

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Dtk    
  Ala41-Ser428 (predicted)    
  Accession # Q06418

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Dtk (also known as Sky, Tyro3, Rse, Brt), Axl (also known as Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor Protein S. The binding affinities for Gas6 are in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

• References:

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.

2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

• Long Name:

  Tyrosine Protein Kinase Receptor TYRO3

• Entrez Gene IDs:

  7301 (Human); 22174 (Mouse)

• Alternate Names:

  Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY