• Species Reactivity

  Human

• Specificity

  Detects human NG2/MCSP in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # LHM-2

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human melanoma cells

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

• References:

1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.

2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.

3. Legg, J. et al. (2003) Development 130:6049.

• Long Name:

  Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

• Entrez Gene IDs:

  1464 (Human); 121021 (Mouse); 81651 (Rat)

• Alternate Names:

  AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8