• Species Reactivity

  Human

• Specificity

  Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF? beta 1, rhTGF? beta 2, rhTGF? beta 1.2, rhTGF? beta 3, or rhTGF? alpha.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 27232

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human LAP (TGF-beta 1)    
  Leu30-Arg278 (Cys33Ser)    
  Accession # P01137

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAP (TGF-beta 1)

The TGF? beta family includes several related proteins (70?80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF? beta 1, TGF? beta 2, TGF? beta 1.2, TGF? beta 3, TGF? beta 4, and TGF? beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF? beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF? beta family members are naturally associated with their own distinct LAPs.

• Long Name:

  Latency-associated Peptide

• Entrez Gene IDs:

  7040 (Human)

• Alternate Names:

  LAP (TGFbeta 1); LAP (TGF-beta 1)