• Species Reactivity

  Human

• Specificity

  Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Nanog    
  Trp153-Val305    
  Accession # ABZ92376

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1?3).

• References:

1. Mitsui, K. et al. (2003) Cell 11:631.

2. Chambers, I. et al. (2003) Cell 113:643.

3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

• Long Name:

  Nanog Homeobox

• Entrez Gene IDs:

  79923 (Human); 71950 (Mouse)

• Alternate Names:

  FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog