• Species Reactivity

  Mouse

• Specificity

  Detects mouse Rae?1 alpha / beta /δ/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.

• Source

  Monoclonal Rat IgG2a Clone # 199205

• Immunogen

  BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58)

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  RAW 264.7 mouse monocyte/macrophage cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88?95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in   in vivo tumor rejection.

• Long Name:

  Retinoic Acid Early Transcript 1

• Entrez Gene IDs:

  8480 (Human); 19368 (Mouse)

• Alternate Names:

  Rae1; Rae-1