• Species Reactivity

  Human

• Specificity

  Detects human IL-37/IL-1F7 in ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 899826

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human IL-37/IL-1F7    
  Lys53-Val206    
  Accession # Q9NZH6

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human peripheral blood mononuclear cell (PBMC) monocytes treated with LPS and monensin fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-37/IL-1F7

Human Interleukin 1 Family Member 7 (IL-1F7), also named IL-37, FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family.  With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1?8).

• References:

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.

2. Kumar S. et al. (2000) J. Biol. Chem. 275:10308.

3. Busfield S.J. et al. (2000) Genomics 66:213.

4. Kumar S. et al. (2002) Cytokine 18:61.

5. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.

6. Gao, W. et al. (2003) J. Immunol. 170:1077.

7. Pan, G. et al. (2001) Cytokine 13:1.

8. Sims, J.E. et al. (2001) Trends Immunol. 22:533.

• Long Name:

  Interleukin 37

• Entrez Gene IDs:

  27178 (Human)

• Alternate Names:

  FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein