• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human Androgen R/NR3C4 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 523339

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Androgen R/NR3C4    
  Thr660-Gln919    
  Accession # P10275

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Androgen R/NR3C4

AR (Androgen Receptor), also known as NR3C4, is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100?120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, macrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N?terminus (aa 1?553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554?635), and a ligand-binding domain (aa 672?917). AR is highly polymorphic at the N?terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1?538 that generates a 45 kDa isoform. AR does homodimerize, apparently with multiple isotypes. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

• Long Name:

  Androgen Receptor

• Entrez Gene IDs:

  367 (Human)

• Alternate Names:

  AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy