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Human Flt-3/Flk-2 Alexa Fluor 700 MAb (Clone 66903) 100 TESTS

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Human Flt-3/Flk-2 Alexa Fluor 700 MAb (Clone 66903) 100 TESTS信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detect human Flt?3/Flk?2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Flt-3, recombinant human (rh) SCF R, rhPDGF R alpha, or rhPDGF R beta is observed.

    • Source

      Monoclonal Mouse IgG    1 Clone # 66903

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant human Flt?3/Flk?2    
      Asn27-Asn541    
      Accession # AAA18947

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 700

    Applications


    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of Flt?3/Flk?2 in THP?1 Human Cell Line by Flow Cytometry. THP?1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human Flt?3/Flk?2 Alexa Fluor? 700?conjugated Monoclonal Antibody (Catalog # FAB812N, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: Flt-3/Flk-2

    The Flt-3 (fms-Like Tyrosine kinase) Receptor, also known as Flk-2 (Fetal Liver Kinase) and Stk-1(Stem cell Tyrosine Kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

    • Long Name:

      fms-like Tyrosine Kinase 3

    • Entrez Gene IDs:

      2322 (Human); 14255 (Mouse)

    • Alternate Names:

      CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3











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