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Human CD160 Allophycocyanin MAb (Clone 688327) 100 TESTS

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Human CD160 Allophycocyanin MAb (Clone 688327) 100 TESTS信息二維碼

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產品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD160 is observed.

    • Source

      Monoclonal Mouse IgG    2B Clone # 688327

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Chinese hamster ovary cell line CHO-derived recombinant human CD160    
      Ile27-Ser159    
      Accession # O95971

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Allophycocyanin

    Applications


    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      10 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of CD160 in Human PBMCs byFlow Cytometry. Human peripheral blood mononuclearcells (PBMCs) were stained with Mouse Anti-Human CD160APC?conjugated Monoclonal Antibody (Catalog # FAB6700A) and MouseAnti-Human
    NCAM?1/CD56 PE?conjugated Monoclonal Antibody(Catalog # ).View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: CD160

    CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules.  It is expressed on select hematopoietic cell types, including CD56  dim CD16  + cytotoxic NK cells, CD8  + CD28  - effector T cells, δ/ gamma T cells, and restricted CD4  + T cells.  It is a receptor for HLA-C molecules, and its engagement induces CD160  + NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program.  Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27?181).  It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V?type Ig-like domain (aa 27-122).  CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182.  The TM form appears to have a splice variant that lacks aa 25-133.  Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.  

    • Entrez Gene IDs:

      11126 (Human); 54215 (Mouse); 502585 (Rat)

    • Alternate Names:

      BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28










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